Many modifications of Eagle's Medium have been developed since the original formulation appeared in the literature.
Among the most widely used of these modifications is Dulbecco's Modified Eagle's medium (DMEM).
The original DMEM formula contains 1000mgj/L of glucose and was first reported for culturing embryonic mouse cells.
A further alteration with 4500 mgj/L glucose has proved to be optimal for cultivation of certain cell types.
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