Cell Culture Supplement and Reagent products:
Growth media is used either for cell culture or microbiological culture. These require nutrients, supplements, buffers and other reagents specific to the product being grown to maximize results and consistency.
Amino acids, vitamins, and HEPES are among the assorted components added to culture media. L-glutamine and and L-alanyl-L-glutamine are used in neural cultures for most select survival. Specialty supplement mixtures are available for specific types of cells, such as MEM amino acids and MEM vitamins.
Trypsin–EDTA solution is used to detach cells from tissue culture dishes and to dissociate cells from one another. Dilute the 10× stock of trypsin–EDTA from the supplier into Ca/Mg-free 1× Dulbecco’s PBS. The final concentrations are 0.05% trypsin, 0.02% (0.53 mM) EDTA.
This diluted solution can be aliquoted (e.g., 5–10 mL) and stored frozen. Ready-to-use 0.05% trypsin, 0.53 mM EDTA is commercially available . It is also possible to use 0.25% trypsin, for routine culture of cells.
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No.
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Product Name
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Content
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Cat. No
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Product Size
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1
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Trypsin-EDTA
(1X) 0.05%
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0.5 g/L of trypsin, 0.2 g/L of EDTA,
With Phenol red
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12-TR1-100
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100 ml
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2
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Trypsin-EDTA
(1X) 0.25%
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2.5 g/L of trypsin, 0.38 g/L of EDTA, With Phenol red
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12-TR2-100
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100 ml
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3
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Trypsin-EDTA
(10X) 0.5%
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5 g/L of trypsin, 2 g/L of EDTA,
Without Phenol red.
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12-TR3-100
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100 ml
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4
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Trypsin-EDTA
(10X) 2.5%
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25 g/L of trypsin, 3.8 g/L of EDTA, Without Phenol red.
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12-TR4-100
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100 ml
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