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Product brief introduction

Buffered Salt Solutions products:

Buffered salt solutions are made to a physiological pH and salt concentration. They are used alone or in combination with other agents for washing tissues and cells. Buffered salt solutions most commonly include sodium, potassium, calcium, magnesium, and chloride. They provide the cells with water and inorganic ions, while maintaining a physiological pH and osmotic pressure.
Although there have been many modifications to the original formulas in efforts to produce fully defined media, salt solutions still play an important role in tissue culture. A salt solution's basic function, to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions, is as valuable today as when it was first developed a century ago.


HBSS, Hank's Balanced Salt Solutions 

Hanks’ Balanced Salt Solution (HBSS) is composed of inorganic salts and supplemented with glucose. The solution may be used to wash cells and tissue and to maintain cells in a viable state. The solution is buffered with phosphate and maintains a physiological pH and osmotic pressure. HBSS is sterile filtered through a 0.2μm membrane.
HBSS may be used as a Calcium (Ca2+) and Magnesium (Mg2+)-Free solution for use in Trypsin solutions or it is available with Calcium and Magnesium salts where required.

It generally serves as a general use isotonic saline solution or buffer for washing cells and tissues (e.g. in chromatographic procedures) where incubation is not necessary. HBSS without Calcium and Magnesium is used in the dissociation process to wash and resuspend cells when the presence of Calcium and Magnesium may inhibit Trypsin activity. 



Product Name


Cat. No

Product Size


HBSS (Hanks)

Without Calcium and Magnesium


100 ml


500 ml


HBSS (Hanks)

Without Calcium, Magnesium

and Phenol red


100 ml


500 ml

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